Journal: The Journal of surgical research

Article Title: Timing of valproic acid in acute lung injury: prevention is the best therapy?

doi: 10.1016/j.jss.2017.06.088

Figure Lengend Snippet: Experimental paradigm of varied timing of administration of valproic acid (VPA) after endotracheal Escherichia coli instillation leading to a severe multilobar gram-negative pneumonia, progressing to ARDS. CFU = colony-forming unit. (Color version of figure is available online.)

Article Snippet: Escherichia coli E coli (serotype 06:K3:H1, strain 19,138, American Type Culture Collection, Manassas, VA) were cultured in Luria broth to the desired concentration and subsequently quantified by optical density and verified by serial dilutions on Luria broth plates.

Techniques:

Journal: The Journal of surgical research

Article Title: Timing of valproic acid in acute lung injury: prevention is the best therapy?

doi: 10.1016/j.jss.2017.06.088

Figure Lengend Snippet: Early histone deacetylase inhibition (≤3 h) improves pulmonary [IL-6 (A) and TNF (B)] and systemic [IL-6 (C)] proinflammatory cytokines. (Each value represents mean ± SEM, * = P < 0.05 comparing Escherichia coli to E coli — VPA). SEM = standard error of mean. (Color version of figure is available online.)

Article Snippet: Escherichia coli E coli (serotype 06:K3:H1, strain 19,138, American Type Culture Collection, Manassas, VA) were cultured in Luria broth to the desired concentration and subsequently quantified by optical density and verified by serial dilutions on Luria broth plates.

Techniques: Histone Deacetylase Assay, Inhibition

Journal: PLoS ONE

Article Title: Roles of interleukin-11 during acute bacterial pneumonia

doi: 10.1371/journal.pone.0221029

Figure Lengend Snippet: Mice were infected with S . pneumoniae either Serotype 3 (A-B) or 19 (C-D) in the presence of anti-IL-11 or IgG control for 24h. BALF was collected and analyzed for A, C) neutrophils and B, D) protein. Significance was tested with T-test. N = 3–5.

Article Snippet: Experimental infections were induced by intratracheal (IT) instillation of 50μl saline with approximately 1–2 x 10 6 CFU Escherichia coli ( E . coli serotype 06:K2:H1; ATCC #19138; ATCC, Manassas, VA), or one of two strains of Streptococcus Pneumoniae (S . pneumoniae serotype 3, ATCC 6303 or serotype 19 EF3030, kindly provided by Dr. Marc Lipsitch, Harvard School of Public Health) into the left bronchus as previously described [ , ].

Techniques: Infection, Control

Journal: Veterinary World

Article Title: Prevalence, antibiotic resistance, and virulence gene profile of Escherichia coli strains shared between food and other sources in Africa: A systematic review

doi: 10.14202/vetworld.2023.2016-2028

Figure Lengend Snippet: Characteristics of eligible research papers.

Article Snippet: , Selim et al . [ ] , 2013 , Food, water and clinical samples , 384 , EMB, SMAC, TSI, EHL, PCR, Serotyping , not conducted , stx1, stx2, eae, hlyA , Egypt.

Techniques: Isolation, Reverse Transcription Polymerase Chain Reaction, Cream, DNA Hybridization, Agglutination